Optimization of a Wet Microalgal Lipid Extraction Procedure for Improved Lipid Recovery for Biofuel and Bioproduct Production
Ashik Sathish, Tyler Marlar, Ronald C. Sims
Methods to convert microalgal biomass to bio-based fuels and chemicals are limited by several, processing and economic hurdles. Research conducted in this study modiﬁed/optimized a previously published procedure capable of extracting transesteriﬁable lipids from wet algal biomass. This optimization resulted in the extraction of 77% of the total transesteriﬁable lipids while reducing the amount of materials and temperature required in the procedure. In addition, characterization of side streams generated demonstrated that: (1) the C/N ratio of the residual biomass or lipid extracted (LE) biomass increased to 54.6 versus 10.1 for the original biomass, (2) the aqueous phase generated contains nitrogen, phosphorous, and carbon, and (3) the solid precipitate phase was composed of up to 11.2 wt% nitrogen (70% protein). The ability to isolate algal lipids and the possibility of utilizing generated side streams as products and/or feedstock material for downstream processes helps promote the algal bioreﬁnery concept.
Sathish, A., Marlar, T. & Sims, R. C. Optimization of a wet microalgal lipid extraction procedure for improved lipid recovery for biofuel and bioproduct production. Bioresource Technology 193, 15–24 (2015).
Microalgae; Wet lipid extraction; Lipid extracted algae (LEA); Protein; Biorefinery